Evaluation of Apoptosis in Collagen-induced Arthritis with I-Annexin V Scintigraphy
نویسندگان
چکیده
Received 3/30/2009; revised 4/24/2009; accepted 4/30/2009. For correspondence and reprints contact: Wan-Yu Lin, M.D., Department of Nuclear Medicine, Taichung Veterans General Hospital. 160 Section 3, ChungGung Road, Taichung 407, Taiwan. Tel: (886)4-23741349, Fax: (886)4-23741348, E-mail:[email protected] Background: Rheumatoid arthritis (RA) is a chronic progressive disease and an autoimmune disorder characterized by joint inflammation due to abnormal accumulation of macrophages and autoreactive T lymphocytes in joints. Apoptosis is considered to be part of the pathogenesis of the disease. The radiolabeled annexin V has been proven effective in detecting apoptotic cells. Recently, the Institute of Nuclear Energy Research (INER) has successfully labeled annexin V with I. In this study, we used INER-produced I-Annexin V for the evaluation of apoptotic status in collagen-induced arthritis (CIA) rats. Methods: Ten Sprague-Dawley (SD) rats were divided into two groups: a control group and a CIA group (five in each group). Bovine type II collagen emulsified with complete Freund’s adjuvant was injected intradermally at the back and tail to induce arthritis in SD rats. Scintigraphy was performed 4 h and 24 h after injection of I-annexin V in both CIA rats and control rats. ROI was selected for the ankle joint and the soft tissue of the thigh of the left rear leg in all rats. Activity of ankle to muscle (A/M ratio) was calculated by computer. Afterwards, rats were sacrificed for H&E, TUNEL and DAPI (diamidino-2-phenylindole) staining. Results: Obvious swelling of the left rear legs was noted in CIA rats. Increased I-annexin V uptake in the ankles and paws was noted in the CIA rats. In contrast, no significant I-annexin V uptake was noted in the ankles of the control rats. The A/M ratios in CIA rats were significantly higher when compared to that in control rats at both 4 h and 24 h after I-annexin V injection. The P values were less than 0.05 using the MannWhitney U test. Using the TUNEL and DAPI stain, apoptotic cells were seen in the ankle joints of the left rear legs in CIA rats but not in the ankle joints of normal rats. Conclusion: I-annexin V produced by INER can be used to evaluate apoptosis in the joints of a CIA rat and may be a potential agent for the in-vivo evaluation of apoptosis in humans.
منابع مشابه
SPIO-Annexin V, a potential probe for MRI detection of radiation induced apoptosis
Background: Finding a suitable method for rapid, accurate and reliable estimation of absorbed dose has high priority in management of the radiation exposed persons. Shortly after radiation exposure, apoptosis is a major detriment in proliferative tissues such as the hematopoietic system. Therefore, quantification of apoptosis in these tissues could be useful for rapid estimation of radiation ex...
متن کاملRadionuclide Imaging of Apoptosis in Malignancies: Promise and Pitfalls of 99mTc-Hynic-rh-Annexin V Imaging
Radionuclide detection of apoptosis with of (99m)Tc-Hynic-rh-Annexin V scintigraphy is an effective tool for in vivo visualisation and monitoring of apoptosis in various malignant tumour. Early therapy-induced increase of the tumour tracer uptake correlates with favourable outcome, whereas stable or decreased uptake correlates with stable disease or tumour progression. Therefore sequential (99m...
متن کاملPossible Involvement of a Specific Cell Surface Receptor for Calprotectin-Induced Apoptosis in Colon Adenocarcinoma and Carcinam Cell Lines (SW742 and HT29/219)
Calprotectin, a calcium-bound protein complex, is abundant in the cytosol of neutrophils. It has been reported that this protein has an apoptotic activity in tumor cells. Since calprotectin increases in colorectal cancer, this study was conducted to investigate, for the first time, the cytotoxicity/apoptotic effect of calprotectin on HT29/219 and SW742 colon carcinoma and adenocarcinoma cell li...
متن کاملPreparation and biodistribution study of 99mTc-EC-Annexin-SPIO as a tracer of radiation induced apoptosis in mice model
Introduction: Apoptosis is a major consequence of ionizing radiation in proliferative tissues and quantification of the apoptotic cells could be helpful for noninvasive assessment and estimation of the radiation absorbed dose. Annexin V conjugated with super paramagnetic iron oxide nanoparticles (ANX-SPIO) is a biological probe for detection of apoptotic cells using magnetic resonance imaging. ...
متن کاملاثر سینرژیستی ایندول تری کربینول با دوکسوروبیسین در افزایش القای آپوپتوز بر رده سلولی لوسمی لنفوبلاستیک حاد (NALM-6)
Background and Aim: Indole-3-carbinol (I3C), found in Brassica species vegetables, exhibits antitumor effects. It has been shown that I3C induces apoptosis in various cell types through inactivation of the nuclear factor-kappa B (NF- k B) pathway. Anthracyclines such as doxorubicin, is widely used in the treatment of hematological malignancies, induce apoptosis in tumor cells via DNA damage and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2009